human bladder epithelial cells Search Results


bladder  (ATCC)
99
ATCC bladder
Bladder, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nontumorigenic human bladder epithelial cells hblepc 938 05a  (Cell Applications Inc)
93
Cell Applications Inc nontumorigenic human bladder epithelial cells hblepc 938 05a
Nontumorigenic Human Bladder Epithelial Cells Hblepc 938 05a, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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synoviocyte basal medium  (Cell Applications Inc)
96
Cell Applications Inc synoviocyte basal medium
Synoviocyte Basal Medium, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human primary epithelial cells  (Cell Applications Inc)
92
Cell Applications Inc human primary epithelial cells
A Western blot analysis of protein expression in primary normal human bladder <t>epithelial</t> cells (HBlEpC) and two human BC cell lines (J82 and T24). B–E Cell proliferation of HBlEpC (B), T24 (C), and basal and luminal subtypes of MIBC and NMIBC cell lines (E) after infection with lentiviruses containing human TFCP2L1 or CDK1 ORFs or TFCP2L1 shRNA (two independent shRNAs; #1 and #2). (D) Ectopic expression or silencing of TFCP2L1 and CDK1 in T24 cells was validated by Western blot analysis. F Tumor sphere formation in T24 cells after TFCP2L1 silencing, ectopic expression of TFCP2L1, or TFCP2L1 and CDK1 co‐expression. Images are shown at ×40 (upper panel) or ×100 (lower panel) magnification. Scale bars = 200 μm. G Tumor sphere formation assay in basal and luminal subtypes of MIBC and NMIBC cell lines with ectopic expression or silencing of TFCP2L1 . The representative images for each cell line are available as Fig A and B. H Clonogenic limiting dilution assay of T24 cells with ectopic expression of TFCP2L1 or CDK1 and TFCP2L1 . I Matrigel invasion assays with the indicated T24 cells. Representative images are shown at ×200 magnification. Scale bars = 100 μm. Data information: All quantitative data are mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 compared with cells transfected with the empty control vector, # P < 0.05, ## P < 0.01, n.s. = not significant. Statistical tests used are as follows: one‐way (H, I) and two‐way ANOVA (B, C, E, F, G) with Bonferroni post hoc tests. Number of biological replicates is n ≥ 4. The exact P ‐values and number of replicates can be found in the source data. Source data are available online for this figure.
Human Primary Epithelial Cells, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human urothelial cell line hblak  (CELLnTEC Advanced Cell Systems AG)
90
CELLnTEC Advanced Cell Systems AG human urothelial cell line hblak
A Western blot analysis of protein expression in primary normal human bladder <t>epithelial</t> cells (HBlEpC) and two human BC cell lines (J82 and T24). B–E Cell proliferation of HBlEpC (B), T24 (C), and basal and luminal subtypes of MIBC and NMIBC cell lines (E) after infection with lentiviruses containing human TFCP2L1 or CDK1 ORFs or TFCP2L1 shRNA (two independent shRNAs; #1 and #2). (D) Ectopic expression or silencing of TFCP2L1 and CDK1 in T24 cells was validated by Western blot analysis. F Tumor sphere formation in T24 cells after TFCP2L1 silencing, ectopic expression of TFCP2L1, or TFCP2L1 and CDK1 co‐expression. Images are shown at ×40 (upper panel) or ×100 (lower panel) magnification. Scale bars = 200 μm. G Tumor sphere formation assay in basal and luminal subtypes of MIBC and NMIBC cell lines with ectopic expression or silencing of TFCP2L1 . The representative images for each cell line are available as Fig A and B. H Clonogenic limiting dilution assay of T24 cells with ectopic expression of TFCP2L1 or CDK1 and TFCP2L1 . I Matrigel invasion assays with the indicated T24 cells. Representative images are shown at ×200 magnification. Scale bars = 100 μm. Data information: All quantitative data are mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 compared with cells transfected with the empty control vector, # P < 0.05, ## P < 0.01, n.s. = not significant. Statistical tests used are as follows: one‐way (H, I) and two‐way ANOVA (B, C, E, F, G) with Bonferroni post hoc tests. Number of biological replicates is n ≥ 4. The exact P ‐values and number of replicates can be found in the source data. Source data are available online for this figure.
Human Urothelial Cell Line Hblak, supplied by CELLnTEC Advanced Cell Systems AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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normal human renal epithelial cells  (Kurabo industries)
90
Kurabo industries normal human renal epithelial cells
A Western blot analysis of protein expression in primary normal human bladder <t>epithelial</t> cells (HBlEpC) and two human BC cell lines (J82 and T24). B–E Cell proliferation of HBlEpC (B), T24 (C), and basal and luminal subtypes of MIBC and NMIBC cell lines (E) after infection with lentiviruses containing human TFCP2L1 or CDK1 ORFs or TFCP2L1 shRNA (two independent shRNAs; #1 and #2). (D) Ectopic expression or silencing of TFCP2L1 and CDK1 in T24 cells was validated by Western blot analysis. F Tumor sphere formation in T24 cells after TFCP2L1 silencing, ectopic expression of TFCP2L1, or TFCP2L1 and CDK1 co‐expression. Images are shown at ×40 (upper panel) or ×100 (lower panel) magnification. Scale bars = 200 μm. G Tumor sphere formation assay in basal and luminal subtypes of MIBC and NMIBC cell lines with ectopic expression or silencing of TFCP2L1 . The representative images for each cell line are available as Fig A and B. H Clonogenic limiting dilution assay of T24 cells with ectopic expression of TFCP2L1 or CDK1 and TFCP2L1 . I Matrigel invasion assays with the indicated T24 cells. Representative images are shown at ×200 magnification. Scale bars = 100 μm. Data information: All quantitative data are mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 compared with cells transfected with the empty control vector, # P < 0.05, ## P < 0.01, n.s. = not significant. Statistical tests used are as follows: one‐way (H, I) and two‐way ANOVA (B, C, E, F, G) with Bonferroni post hoc tests. Number of biological replicates is n ≥ 4. The exact P ‐values and number of replicates can be found in the source data. Source data are available online for this figure.
Normal Human Renal Epithelial Cells, supplied by Kurabo industries, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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immortal ureteral epithelium cell sv-huc-1  (iCell Bioscience Inc)
90
iCell Bioscience Inc immortal ureteral epithelium cell sv-huc-1
A Western blot analysis of protein expression in primary normal human bladder <t>epithelial</t> cells (HBlEpC) and two human BC cell lines (J82 and T24). B–E Cell proliferation of HBlEpC (B), T24 (C), and basal and luminal subtypes of MIBC and NMIBC cell lines (E) after infection with lentiviruses containing human TFCP2L1 or CDK1 ORFs or TFCP2L1 shRNA (two independent shRNAs; #1 and #2). (D) Ectopic expression or silencing of TFCP2L1 and CDK1 in T24 cells was validated by Western blot analysis. F Tumor sphere formation in T24 cells after TFCP2L1 silencing, ectopic expression of TFCP2L1, or TFCP2L1 and CDK1 co‐expression. Images are shown at ×40 (upper panel) or ×100 (lower panel) magnification. Scale bars = 200 μm. G Tumor sphere formation assay in basal and luminal subtypes of MIBC and NMIBC cell lines with ectopic expression or silencing of TFCP2L1 . The representative images for each cell line are available as Fig A and B. H Clonogenic limiting dilution assay of T24 cells with ectopic expression of TFCP2L1 or CDK1 and TFCP2L1 . I Matrigel invasion assays with the indicated T24 cells. Representative images are shown at ×200 magnification. Scale bars = 100 μm. Data information: All quantitative data are mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 compared with cells transfected with the empty control vector, # P < 0.05, ## P < 0.01, n.s. = not significant. Statistical tests used are as follows: one‐way (H, I) and two‐way ANOVA (B, C, E, F, G) with Bonferroni post hoc tests. Number of biological replicates is n ≥ 4. The exact P ‐values and number of replicates can be found in the source data. Source data are available online for this figure.
Immortal Ureteral Epithelium Cell Sv Huc 1, supplied by iCell Bioscience Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human bladder epithelial progenitor cells  (CELLnTEC Advanced Cell Systems AG)
90
CELLnTEC Advanced Cell Systems AG human bladder epithelial progenitor cells
A Western blot analysis of protein expression in primary normal human bladder <t>epithelial</t> cells (HBlEpC) and two human BC cell lines (J82 and T24). B–E Cell proliferation of HBlEpC (B), T24 (C), and basal and luminal subtypes of MIBC and NMIBC cell lines (E) after infection with lentiviruses containing human TFCP2L1 or CDK1 ORFs or TFCP2L1 shRNA (two independent shRNAs; #1 and #2). (D) Ectopic expression or silencing of TFCP2L1 and CDK1 in T24 cells was validated by Western blot analysis. F Tumor sphere formation in T24 cells after TFCP2L1 silencing, ectopic expression of TFCP2L1, or TFCP2L1 and CDK1 co‐expression. Images are shown at ×40 (upper panel) or ×100 (lower panel) magnification. Scale bars = 200 μm. G Tumor sphere formation assay in basal and luminal subtypes of MIBC and NMIBC cell lines with ectopic expression or silencing of TFCP2L1 . The representative images for each cell line are available as Fig A and B. H Clonogenic limiting dilution assay of T24 cells with ectopic expression of TFCP2L1 or CDK1 and TFCP2L1 . I Matrigel invasion assays with the indicated T24 cells. Representative images are shown at ×200 magnification. Scale bars = 100 μm. Data information: All quantitative data are mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 compared with cells transfected with the empty control vector, # P < 0.05, ## P < 0.01, n.s. = not significant. Statistical tests used are as follows: one‐way (H, I) and two‐way ANOVA (B, C, E, F, G) with Bonferroni post hoc tests. Number of biological replicates is n ≥ 4. The exact P ‐values and number of replicates can be found in the source data. Source data are available online for this figure.
Human Bladder Epithelial Progenitor Cells, supplied by CELLnTEC Advanced Cell Systems AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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normal human bladder epithelial cells (hbep cells)  (ZenBio)
90
ZenBio normal human bladder epithelial cells (hbep cells)
A Western blot analysis of protein expression in primary normal human bladder <t>epithelial</t> cells (HBlEpC) and two human BC cell lines (J82 and T24). B–E Cell proliferation of HBlEpC (B), T24 (C), and basal and luminal subtypes of MIBC and NMIBC cell lines (E) after infection with lentiviruses containing human TFCP2L1 or CDK1 ORFs or TFCP2L1 shRNA (two independent shRNAs; #1 and #2). (D) Ectopic expression or silencing of TFCP2L1 and CDK1 in T24 cells was validated by Western blot analysis. F Tumor sphere formation in T24 cells after TFCP2L1 silencing, ectopic expression of TFCP2L1, or TFCP2L1 and CDK1 co‐expression. Images are shown at ×40 (upper panel) or ×100 (lower panel) magnification. Scale bars = 200 μm. G Tumor sphere formation assay in basal and luminal subtypes of MIBC and NMIBC cell lines with ectopic expression or silencing of TFCP2L1 . The representative images for each cell line are available as Fig A and B. H Clonogenic limiting dilution assay of T24 cells with ectopic expression of TFCP2L1 or CDK1 and TFCP2L1 . I Matrigel invasion assays with the indicated T24 cells. Representative images are shown at ×200 magnification. Scale bars = 100 μm. Data information: All quantitative data are mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 compared with cells transfected with the empty control vector, # P < 0.05, ## P < 0.01, n.s. = not significant. Statistical tests used are as follows: one‐way (H, I) and two‐way ANOVA (B, C, E, F, G) with Bonferroni post hoc tests. Number of biological replicates is n ≥ 4. The exact P ‐values and number of replicates can be found in the source data. Source data are available online for this figure.
Normal Human Bladder Epithelial Cells (Hbep Cells), supplied by ZenBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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normal human bladder epithelial cells (hbep cells) - by Bioz Stars, 2026-02
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human bladder epithelial cells from the dome region (hbec-d)  (Lifeline Cell Technology)
90
Lifeline Cell Technology human bladder epithelial cells from the dome region (hbec-d)
A Western blot analysis of protein expression in primary normal human bladder <t>epithelial</t> cells (HBlEpC) and two human BC cell lines (J82 and T24). B–E Cell proliferation of HBlEpC (B), T24 (C), and basal and luminal subtypes of MIBC and NMIBC cell lines (E) after infection with lentiviruses containing human TFCP2L1 or CDK1 ORFs or TFCP2L1 shRNA (two independent shRNAs; #1 and #2). (D) Ectopic expression or silencing of TFCP2L1 and CDK1 in T24 cells was validated by Western blot analysis. F Tumor sphere formation in T24 cells after TFCP2L1 silencing, ectopic expression of TFCP2L1, or TFCP2L1 and CDK1 co‐expression. Images are shown at ×40 (upper panel) or ×100 (lower panel) magnification. Scale bars = 200 μm. G Tumor sphere formation assay in basal and luminal subtypes of MIBC and NMIBC cell lines with ectopic expression or silencing of TFCP2L1 . The representative images for each cell line are available as Fig A and B. H Clonogenic limiting dilution assay of T24 cells with ectopic expression of TFCP2L1 or CDK1 and TFCP2L1 . I Matrigel invasion assays with the indicated T24 cells. Representative images are shown at ×200 magnification. Scale bars = 100 μm. Data information: All quantitative data are mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 compared with cells transfected with the empty control vector, # P < 0.05, ## P < 0.01, n.s. = not significant. Statistical tests used are as follows: one‐way (H, I) and two‐way ANOVA (B, C, E, F, G) with Bonferroni post hoc tests. Number of biological replicates is n ≥ 4. The exact P ‐values and number of replicates can be found in the source data. Source data are available online for this figure.
Human Bladder Epithelial Cells From The Dome Region (Hbec D), supplied by Lifeline Cell Technology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human svhuc-1 bladder epithelial cells hucl-022  (iCell Bioscience Inc)
90
iCell Bioscience Inc human svhuc-1 bladder epithelial cells hucl-022
A Western blot analysis of protein expression in primary normal human bladder <t>epithelial</t> cells (HBlEpC) and two human BC cell lines (J82 and T24). B–E Cell proliferation of HBlEpC (B), T24 (C), and basal and luminal subtypes of MIBC and NMIBC cell lines (E) after infection with lentiviruses containing human TFCP2L1 or CDK1 ORFs or TFCP2L1 shRNA (two independent shRNAs; #1 and #2). (D) Ectopic expression or silencing of TFCP2L1 and CDK1 in T24 cells was validated by Western blot analysis. F Tumor sphere formation in T24 cells after TFCP2L1 silencing, ectopic expression of TFCP2L1, or TFCP2L1 and CDK1 co‐expression. Images are shown at ×40 (upper panel) or ×100 (lower panel) magnification. Scale bars = 200 μm. G Tumor sphere formation assay in basal and luminal subtypes of MIBC and NMIBC cell lines with ectopic expression or silencing of TFCP2L1 . The representative images for each cell line are available as Fig A and B. H Clonogenic limiting dilution assay of T24 cells with ectopic expression of TFCP2L1 or CDK1 and TFCP2L1 . I Matrigel invasion assays with the indicated T24 cells. Representative images are shown at ×200 magnification. Scale bars = 100 μm. Data information: All quantitative data are mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 compared with cells transfected with the empty control vector, # P < 0.05, ## P < 0.01, n.s. = not significant. Statistical tests used are as follows: one‐way (H, I) and two‐way ANOVA (B, C, E, F, G) with Bonferroni post hoc tests. Number of biological replicates is n ≥ 4. The exact P ‐values and number of replicates can be found in the source data. Source data are available online for this figure.
Human Svhuc 1 Bladder Epithelial Cells Hucl 022, supplied by iCell Bioscience Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hum-u007  (iCell Bioscience Inc)
90
iCell Bioscience Inc hum-u007
A Western blot analysis of protein expression in primary normal human bladder <t>epithelial</t> cells (HBlEpC) and two human BC cell lines (J82 and T24). B–E Cell proliferation of HBlEpC (B), T24 (C), and basal and luminal subtypes of MIBC and NMIBC cell lines (E) after infection with lentiviruses containing human TFCP2L1 or CDK1 ORFs or TFCP2L1 shRNA (two independent shRNAs; #1 and #2). (D) Ectopic expression or silencing of TFCP2L1 and CDK1 in T24 cells was validated by Western blot analysis. F Tumor sphere formation in T24 cells after TFCP2L1 silencing, ectopic expression of TFCP2L1, or TFCP2L1 and CDK1 co‐expression. Images are shown at ×40 (upper panel) or ×100 (lower panel) magnification. Scale bars = 200 μm. G Tumor sphere formation assay in basal and luminal subtypes of MIBC and NMIBC cell lines with ectopic expression or silencing of TFCP2L1 . The representative images for each cell line are available as Fig A and B. H Clonogenic limiting dilution assay of T24 cells with ectopic expression of TFCP2L1 or CDK1 and TFCP2L1 . I Matrigel invasion assays with the indicated T24 cells. Representative images are shown at ×200 magnification. Scale bars = 100 μm. Data information: All quantitative data are mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 compared with cells transfected with the empty control vector, # P < 0.05, ## P < 0.01, n.s. = not significant. Statistical tests used are as follows: one‐way (H, I) and two‐way ANOVA (B, C, E, F, G) with Bonferroni post hoc tests. Number of biological replicates is n ≥ 4. The exact P ‐values and number of replicates can be found in the source data. Source data are available online for this figure.
Hum U007, supplied by iCell Bioscience Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A Western blot analysis of protein expression in primary normal human bladder epithelial cells (HBlEpC) and two human BC cell lines (J82 and T24). B–E Cell proliferation of HBlEpC (B), T24 (C), and basal and luminal subtypes of MIBC and NMIBC cell lines (E) after infection with lentiviruses containing human TFCP2L1 or CDK1 ORFs or TFCP2L1 shRNA (two independent shRNAs; #1 and #2). (D) Ectopic expression or silencing of TFCP2L1 and CDK1 in T24 cells was validated by Western blot analysis. F Tumor sphere formation in T24 cells after TFCP2L1 silencing, ectopic expression of TFCP2L1, or TFCP2L1 and CDK1 co‐expression. Images are shown at ×40 (upper panel) or ×100 (lower panel) magnification. Scale bars = 200 μm. G Tumor sphere formation assay in basal and luminal subtypes of MIBC and NMIBC cell lines with ectopic expression or silencing of TFCP2L1 . The representative images for each cell line are available as Fig A and B. H Clonogenic limiting dilution assay of T24 cells with ectopic expression of TFCP2L1 or CDK1 and TFCP2L1 . I Matrigel invasion assays with the indicated T24 cells. Representative images are shown at ×200 magnification. Scale bars = 100 μm. Data information: All quantitative data are mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 compared with cells transfected with the empty control vector, # P < 0.05, ## P < 0.01, n.s. = not significant. Statistical tests used are as follows: one‐way (H, I) and two‐way ANOVA (B, C, E, F, G) with Bonferroni post hoc tests. Number of biological replicates is n ≥ 4. The exact P ‐values and number of replicates can be found in the source data. Source data are available online for this figure.

Journal: EMBO Molecular Medicine

Article Title: Phosphorylation of TFCP2L1 by CDK1 is required for stem cell pluripotency and bladder carcinogenesis

doi: 10.15252/emmm.201910880

Figure Lengend Snippet: A Western blot analysis of protein expression in primary normal human bladder epithelial cells (HBlEpC) and two human BC cell lines (J82 and T24). B–E Cell proliferation of HBlEpC (B), T24 (C), and basal and luminal subtypes of MIBC and NMIBC cell lines (E) after infection with lentiviruses containing human TFCP2L1 or CDK1 ORFs or TFCP2L1 shRNA (two independent shRNAs; #1 and #2). (D) Ectopic expression or silencing of TFCP2L1 and CDK1 in T24 cells was validated by Western blot analysis. F Tumor sphere formation in T24 cells after TFCP2L1 silencing, ectopic expression of TFCP2L1, or TFCP2L1 and CDK1 co‐expression. Images are shown at ×40 (upper panel) or ×100 (lower panel) magnification. Scale bars = 200 μm. G Tumor sphere formation assay in basal and luminal subtypes of MIBC and NMIBC cell lines with ectopic expression or silencing of TFCP2L1 . The representative images for each cell line are available as Fig A and B. H Clonogenic limiting dilution assay of T24 cells with ectopic expression of TFCP2L1 or CDK1 and TFCP2L1 . I Matrigel invasion assays with the indicated T24 cells. Representative images are shown at ×200 magnification. Scale bars = 100 μm. Data information: All quantitative data are mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 compared with cells transfected with the empty control vector, # P < 0.05, ## P < 0.01, n.s. = not significant. Statistical tests used are as follows: one‐way (H, I) and two‐way ANOVA (B, C, E, F, G) with Bonferroni post hoc tests. Number of biological replicates is n ≥ 4. The exact P ‐values and number of replicates can be found in the source data. Source data are available online for this figure.

Article Snippet: In addition, human primary epithelial cells derived from normal human bladder (HBlEpC; Cell Applications, Inc, San Diego, CA) and human BC cell lines J82, T24, 5637, HT1197, HT1376, and RT4 (purchased from ATCC, Manassas, VA) were employed.

Techniques: Western Blot, Expressing, Infection, shRNA, Tube Formation Assay, Limiting Dilution Assay, Transfection, Control, Plasmid Preparation